97 Search Results for "gel and acrylamide and agarose"

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Origin of life and living matter in hot mineral water, Prof. I. Ignatov, Dr. O. Mosin

https://benchfly.com/video/3445/origin-of-life-and-living-matter-in-hot-miner/

Origin of life and living matter in hot mineral water, Prof. I. Ignatov, Dr. O. Mosin. Water for origination of life. “Water on Earth has contained more deuterium molecules according to Dr. Mosin”. Previous biological experiments with heavy water and structural-conformational studies with deuterated molecules, performed by us, enable to modeling conditions under which the first living forms of life might be evolved (Ignatov & Mosin, 2013a; Ignatov & Mosin, 2013b; Ignatov & Mosin, 2013c). The content of deuterium in hot mineral water may be increased due to the physical chemical processes of the deuterium accumulation. It can be presumed that primary water might contain more deuterium at early stages of evolution of first living structures, and deuterium was distributed non-uniformly in the hydrosphere and atmosphere (Ignatov & Mosin, 2012). The primary reductive atmosphere of the Earth consisted basically of gas mixture CO, H2, N2, NH3, CH4, lacked O2–O3 layer protecting the Earth surface from rigid short-wave solar radiation carrying huge energy capable to cause radiolysis and photolysis of water. The processes accompanying accumulation of deuterium in the hydrosphere are solar radiation, volcanic geothermal processes and electric discharges in the atmosphere. These natural processes could lead to the enrichment of the hydrosphere by deuterium in the form of HDO which evaporates more slowly than H2O, and condenses faster. If this is true, this is a significant fact regarding thermal stability of deuterated macromolecules in the preservation of life under thermal conditions, because chemical bonds with participation of deuterium are stronger than those ones formed of hydrogen. Natural prevalence of deuterium makes up approximately 0.015–0.020 at.%, and depends strongly on the uniformity of substance and the total amount of matter formed in the course of early Galaxy evolution (Linsky, 2007). Constant sources of deuterium are explosions of nova stars and thermonuclear processes frequently occurring inside the stars. Probably, it could explain a known fact, why the amount of deuterium is slightly increased during the global changes of climate in warming conditions. The gravitational field of the Earth is insufficiently strong for the retaining of lighter hydrogen, and our planet is gradually losing hydrogen as a result of its dissociation into interplanetary space. Hydrogen evaporates faster than heavy deuterium, which can be collected by the hydrosphere. Therefore, as a result of this natural process of fractionation of H/D isotopes throughout the process of Earth evolution there should be an accumulation of deuterium in the hydrosphere and surface waters, while in the atmosphere and in water vapour deuterium content tends to be low. Thus, on the planet there occurs a natural process of separation of H and D isotopes, playing an essential role in the maintenance of life on the planet. The second point regards the influence of temperature on the biochemical processes in living matter. Recent studies have shown that the most favorable for the origin of life and living matter seem to be hot alkaline mineral waters interacting with CaCO3 (Ignatov, 2010; Ignatov & Mosin, 2013d). According to the law for conservation of energy the process of self-organization of primary organic forms in water solutions may be supported by thermal energy of magma, volcanic activity and solar radiation. The accumulation of organic compounds in open lakes is more possible compared to the ocean. Life began near a hydrothermal vent: an underwater spout of hot water. Geothermal activity gives more opportunities for the origination of life. The origination of living matter most probably occurred in hot mineral water. This occurred in ponds and hydrothermal vents in seawater or hot mineral water. An indisputable proof of this is the presence of stromatolites fossils. They lived in warm and hot water in zones of volcanic activity, which could be heated by magma and seem to be more stable than other first sea organisms.

Tagged as: origin of life, living matter, hot mineral water, deuterium, heavy water, first organic forms of life, stromatolites, Ignat Ignatov, Oleg Mosin

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Growth Factor and Stem Cell Reagents

https://benchfly.com/video/814/growth-factor-and-stem-cell-reagents/

Goldbio launches a new line of Growth Factors and Stem Cell Reagents This video provides information on growth factors, or, recombinant proteins, or, cytokines, also referred to as stem cell culture and neuronal culture. This is a new product offering from GoldBio. Announcing an exciting development at GoldBio. We have recently launched our newest product, a Recombinant Protein. Growth factors, or cytokines are a type of stem cell and neuronal culture used in many research applications as it relates to the very cellular building blocks. A downloadable PDF on the Growth Factor can be found here: http://bit.ly/VbqDSn To begin, we have over 35 widely used growth factors available, most notably FGF2 or basic FGF. It is complimented by many other human, rat, and murine growth factors such as BMP, CSF, EGF, EPO, FGF, GDNF, IGF, Noggin, NTF, SHH, VEGF.

Tagged as: cellular growth factors, cytokines and growth factors, cytokines growth factors, growth factor, growth factor pathway, growth factor receptor, growth factor receptors, growth factor signaling, growth factors, growth factors and cytokines, recombinant growth factor, stem cell growth factor, recombinant protein, GoldBio

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Graphing your standards from a protein gel

https://benchfly.com/video/1536/graphing-your-standards-from-a-protein-gel/

Basically, how do you create a standard sizing curve from a protein gel. You need to measure the mobility of your bands and graph them. The curve fit is certainly things people argue over, as the relationship is really sigmodial and doesn't fit very well at the extremes but try out different fits and prove to yourself that exponential and power fits are very similar in output.

Tagged as: standard curve, protein gel, ladder, markers, size markers, standards

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Pouring and Running an Agarose Gel

https://benchfly.com/video/91/pouring-and-running-an-agarose-gel/

Knowing how to pour and run DNA agarose gel electrophoresis is essential for many molecular biology techniques. Here I show how to pour agarose gels in two of the most common types of gel boxes.

Tagged as: dna, molecular biology, agar, biology, biochemistry, pcr, cloning,

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Gel Red vs EtBr

https://benchfly.com/video/158/gel-red-vs-etbr/

Here we compare the DNA stains Gel-red vs. EtBr head to head. The Gel-red is in sample whereas the EtBr is in gel. All conditions are the same except for the DNA stains. I am using 1X TAE buffer and a VWR agarose.

Tagged as: Kirkwood, Community, College, Greg, Petersen, Biotech, electrophoresis, Gel-red, Ethidium, Bromide, DNA, Ladder, NEB, Owl

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How to Pour an Agarose Column

https://benchfly.com/video/731/how-to-pour-an-agarose-column/

Pouring an agarose gel may not be the most exciting protocol in the world, but it's a key technique in the lab. Here we show you how to pour highly reproducible gels for technically demanding experiments. We also give tips on shortcuts you can take when pouring routine gels, where precision isn't critical. Buying resin in bulk and packing yourself is fast and is an easy way to save money.

Tagged as: column, agarose, protein, GoldBio, DNA

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ROKEPIE ® - How to store or transport cells and tissue at 2-8 degrees C

https://benchfly.com/video/2634/rokepie-how-to-store-or-transport-cells-and-t/

Learn more about hypothermic preservation. This video shows you how to preserve cells & tissue at 2-8 degrees C. in the fridge. Ideal when you need to "pause" cells during the weekend, transport research material to another facility or want to extend shelf-life of your cells. ROKEPIE®is a new and innovative preservation product for all short-term preservation demands. It is non-toxic and very user-friendly. Just add, incubate, store, rewarm and re-use!

Tagged as: hypothermic, cryostorage, cells, tissue, cell based assays, stem cells, research, for Research Use Only, extend shelf-life, cold chain transportation, preservation, non-toxic, cryopreservation, alternative, user-friendly, cell storage, preservation solution, non-dmso

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OpenWetWare Open Notebook

https://benchfly.com/video/173/openwetware-open-notebook/

Open Notebook Science is an up and coming aspect to working in the lab. Every lab has their own method of sharing research and we at the KochLab use OpenWetWare.org's notebook feature to make all of our protocols and data public. Here is how I organize and share my notes using all the web has to offer.

Tagged as: openwetware, open notebook, open notebook science, kochlab,

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Tip for Loading Many Gel Samples Quickly

https://benchfly.com/video/85/tip-for-loading-many-gel-samples-quickly/

I use this technique for loading many gel samples quickly when genotyping and running gels on large numbers of samples. It makes adding the loading dye to all of them very easy.

Tagged as: shortcut, timesaver, trick, agarose, dna, genotyping, biology, molecular biology, biochemistry, pcr, cloning, agarose, acrylamide

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Restriction Enzyme Digest Problem: Too Many DNA Bands

https://benchfly.com/video/1538/restriction-enzyme-digest-problem-too-many-dn/

It has happened to us all; you run a gel expecting one or two bands, but end up with additional, unexpected bands of varying size and intensity. Typically, these off-target bands are caused by either star activity or partial digestion. Yvette explains both of these phenomena, and gives tips for avoiding them in the future, including optimizing incubation time and using NEB’s High-Fidelity (HF®) Restriction Enzymes.

Tagged as: troubleshooting, molecular biology, cloning,

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