98 Search Results for "pipetman and pipette and pipet"

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Origin of life and living matter in hot mineral water, Prof. I. Ignatov, Dr. O. Mosin

https://benchfly.com/video/3445/origin-of-life-and-living-matter-in-hot-miner/

Origin of life and living matter in hot mineral water, Prof. I. Ignatov, Dr. O. Mosin. Water for origination of life. “Water on Earth has contained more deuterium molecules according to Dr. Mosin”. Previous biological experiments with heavy water and structural-conformational studies with deuterated molecules, performed by us, enable to modeling conditions under which the first living forms of life might be evolved (Ignatov & Mosin, 2013a; Ignatov & Mosin, 2013b; Ignatov & Mosin, 2013c). The content of deuterium in hot mineral water may be increased due to the physical chemical processes of the deuterium accumulation. It can be presumed that primary water might contain more deuterium at early stages of evolution of first living structures, and deuterium was distributed non-uniformly in the hydrosphere and atmosphere (Ignatov & Mosin, 2012). The primary reductive atmosphere of the Earth consisted basically of gas mixture CO, H2, N2, NH3, CH4, lacked O2–O3 layer protecting the Earth surface from rigid short-wave solar radiation carrying huge energy capable to cause radiolysis and photolysis of water. The processes accompanying accumulation of deuterium in the hydrosphere are solar radiation, volcanic geothermal processes and electric discharges in the atmosphere. These natural processes could lead to the enrichment of the hydrosphere by deuterium in the form of HDO which evaporates more slowly than H2O, and condenses faster. If this is true, this is a significant fact regarding thermal stability of deuterated macromolecules in the preservation of life under thermal conditions, because chemical bonds with participation of deuterium are stronger than those ones formed of hydrogen. Natural prevalence of deuterium makes up approximately 0.015–0.020 at.%, and depends strongly on the uniformity of substance and the total amount of matter formed in the course of early Galaxy evolution (Linsky, 2007). Constant sources of deuterium are explosions of nova stars and thermonuclear processes frequently occurring inside the stars. Probably, it could explain a known fact, why the amount of deuterium is slightly increased during the global changes of climate in warming conditions. The gravitational field of the Earth is insufficiently strong for the retaining of lighter hydrogen, and our planet is gradually losing hydrogen as a result of its dissociation into interplanetary space. Hydrogen evaporates faster than heavy deuterium, which can be collected by the hydrosphere. Therefore, as a result of this natural process of fractionation of H/D isotopes throughout the process of Earth evolution there should be an accumulation of deuterium in the hydrosphere and surface waters, while in the atmosphere and in water vapour deuterium content tends to be low. Thus, on the planet there occurs a natural process of separation of H and D isotopes, playing an essential role in the maintenance of life on the planet. The second point regards the influence of temperature on the biochemical processes in living matter. Recent studies have shown that the most favorable for the origin of life and living matter seem to be hot alkaline mineral waters interacting with CaCO3 (Ignatov, 2010; Ignatov & Mosin, 2013d). According to the law for conservation of energy the process of self-organization of primary organic forms in water solutions may be supported by thermal energy of magma, volcanic activity and solar radiation. The accumulation of organic compounds in open lakes is more possible compared to the ocean. Life began near a hydrothermal vent: an underwater spout of hot water. Geothermal activity gives more opportunities for the origination of life. The origination of living matter most probably occurred in hot mineral water. This occurred in ponds and hydrothermal vents in seawater or hot mineral water. An indisputable proof of this is the presence of stromatolites fossils. They lived in warm and hot water in zones of volcanic activity, which could be heated by magma and seem to be more stable than other first sea organisms.

Tagged as: origin of life, living matter, hot mineral water, deuterium, heavy water, first organic forms of life, stromatolites, Ignat Ignatov, Oleg Mosin

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Growth Factor and Stem Cell Reagents

https://benchfly.com/video/814/growth-factor-and-stem-cell-reagents/

Goldbio launches a new line of Growth Factors and Stem Cell Reagents This video provides information on growth factors, or, recombinant proteins, or, cytokines, also referred to as stem cell culture and neuronal culture. This is a new product offering from GoldBio. Announcing an exciting development at GoldBio. We have recently launched our newest product, a Recombinant Protein. Growth factors, or cytokines are a type of stem cell and neuronal culture used in many research applications as it relates to the very cellular building blocks. A downloadable PDF on the Growth Factor can be found here: http://bit.ly/VbqDSn To begin, we have over 35 widely used growth factors available, most notably FGF2 or basic FGF. It is complimented by many other human, rat, and murine growth factors such as BMP, CSF, EGF, EPO, FGF, GDNF, IGF, Noggin, NTF, SHH, VEGF.

Tagged as: cellular growth factors, cytokines and growth factors, cytokines growth factors, growth factor, growth factor pathway, growth factor receptor, growth factor receptors, growth factor signaling, growth factors, growth factors and cytokines, recombinant growth factor, stem cell growth factor, recombinant protein, GoldBio

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Micropipette Calibration and Analysis

https://benchfly.com/video/112/micropipette-calibration-and-analysis/

Proper micropipette calibration is critically important and should be performed annually to ensure volumes are accurate. Over time, pipette volumes tend to drift so the more time passes after calibration, the larger the odds that the pipette is not picking up or dispensing the desired volume. Therefore, micropipette calibration can directly impact the reproducibility of experiments and the quality of the resulting data.

Tagged as: micro pipette, kirkwood, greg petersen, calibration, spreadsheet, accuracy, precision, analytical balance

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How to Make a Bacterial Spreader

https://benchfly.com/video/65/how-to-make-a-bacterial-spreader/

Making your own bacterial spreader is fast and easy as we demonstrate in this video - all you need is a pipette and a flame. No words necessary...it's that easy!

Tagged as: pasteur pipet, glass pipet, pipette, spreader, transformation, science videos, current protocols, laboratory techniques, benchfly, biology, biochemistry, molecular biology, bacteria

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ROKEPIE ® - How to store or transport cells and tissue at 2-8 degrees C

https://benchfly.com/video/2634/rokepie-how-to-store-or-transport-cells-and-t/

Learn more about hypothermic preservation. This video shows you how to preserve cells & tissue at 2-8 degrees C. in the fridge. Ideal when you need to "pause" cells during the weekend, transport research material to another facility or want to extend shelf-life of your cells. ROKEPIE®is a new and innovative preservation product for all short-term preservation demands. It is non-toxic and very user-friendly. Just add, incubate, store, rewarm and re-use!

Tagged as: hypothermic, cryostorage, cells, tissue, cell based assays, stem cells, research, for Research Use Only, extend shelf-life, cold chain transportation, preservation, non-toxic, cryopreservation, alternative, user-friendly, cell storage, preservation solution, non-dmso

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OpenWetWare Open Notebook

https://benchfly.com/video/173/openwetware-open-notebook/

Open Notebook Science is an up and coming aspect to working in the lab. Every lab has their own method of sharing research and we at the KochLab use OpenWetWare.org's notebook feature to make all of our protocols and data public. Here is how I organize and share my notes using all the web has to offer.

Tagged as: openwetware, open notebook, open notebook science, kochlab,

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Graphing your standards from a protein gel

https://benchfly.com/video/1536/graphing-your-standards-from-a-protein-gel/

Basically, how do you create a standard sizing curve from a protein gel. You need to measure the mobility of your bands and graph them. The curve fit is certainly things people argue over, as the relationship is really sigmodial and doesn't fit very well at the extremes but try out different fits and prove to yourself that exponential and power fits are very similar in output.

Tagged as: standard curve, protein gel, ladder, markers, size markers, standards

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Your Pipette and You, Part I

https://benchfly.com/video/175/your-pipette-and-you-part-i/

The micropipettor is one of the most used tools in the biochemist's and molecular biologist's toolbox. Learn to use it properly and your data will be so much better!

Tagged as: Pipetting, Lab Techniques

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How to Use a Pipetman

https://benchfly.com/video/151/how-to-use-a-pipetman/

Understanding the basic principles of how to use a pipetman is an essential part of biological research. Here's how to select the proper pipetman, load samples, and dispense liquid.

Tagged as: pipetman, pipetteman, laboratory techniques, science videos, current protocols, biology, biochemistry, introduction, beginner, molecular biology

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How to Read Volume on a Pipetman

https://benchfly.com/video/152/how-to-read-volume-on-a-pipetman/

Reading volume on a pipetman will eventually become second nature, but when first starting out, it can be easy to get tripped up. Here's a quick tutorial on reading the volume in P20, P200 and P1000 pipetman.

Tagged as: P20, P200, P100, pipetman, laboratory techniques, science videos, current protocols, micropipette, biology, biochemistry, molecular biology

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