Why is My Restriction Enzyme Not Cutting DNA?

New England Biolabs


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There are a number of reasons that your restriction enzyme might not digest your DNA as expected, beyond having an expired lot of endonuclease. Before you toss that tube of enzyme, let Yvette walk you through some of the most common causes of unsuccessful digestion reactions. She’ll explain the control experiments for determining whether or not your samples contain contaminants, as well as tips on how to assess the methylation status of your DNA.