Saved!

What Causes a Difficult Ligation Reaction

New England Biolabs

5082

Answer Questions Below

No Feedback Form Available for this Video

If your DNA fragments have blunt ends or single-base overhangs, you’ll want to watch as Greg explains why they can be difficult to ligate, and how you can improve the outcomes of your ligation reactions. A few simple tips can help you to overcome the challenges posed by ligation of non-cohesive ends.