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Why Should You Use PEG in a Ligation Reaction?

New England Biolabs

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Have you been adding PEG to your ligase reactions, but weren’t entirely sure why? Or did someone recommend the addition of PEG to improve your ligations, but you’d like a little background on why it might help? Greg can explain the hows and whys of PEG’s addition to ligation reactions, and help you to decide whether or not it might be right for your ligations.