What is the Best Ratio of Reactants in a Ligation?

New England Biolabs


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1:3? 1:1? 3:1? Which molar ratio of insert:vector is your best bet? Eric explains why a 3:1 insert:vector molar ratio is commonly recommended for general subcloning, while a molar ratio from 10:1 to 50:1 is recommended for adaptor ligation.