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How to use a Semi-dry Transfer Apparatus

Alan Marnett

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A semi-dry transfer apparatus is one of the easiest ways to transfer proteins to nitrocellulose prior to a western blot. The semi-dry transfer has several advantages over a traditional wet transfer including the ease of setup, minimal solvent required, and higher throughput.

After using the semi-dry apparatus, I'll never go back to the wet transfer again! See attached file under video for Transfer Buffer Recipe.