16 Search Results for "tips" – Page 2
Restriction Enzyme Digest Problem: Too Many DNA Bands
https://benchfly.com/video/1538/restriction-enzyme-digest-problem-too-many-dn/It has happened to us all; you run a gel expecting one or two bands, but end up with additional, unexpected bands of varying size and intensity. Typically, these off-target bands are caused by either star activity or partial digestion. Yvette explains both of these phenomena, and gives tips for avoiding them in the future, including optimizing incubation time and using NEB’s High-Fidelity (HF®) Restriction Enzymes.
Restriction Enzyme Digest Problem: Smear on Agarose Gel
https://benchfly.com/video/1540/restriction-enzyme-digest-problem-smear-on-ag/You’re expecting a band at 89 kD, but all you see is an uninterpretable smear when you analyze your agarose gel. What went wrong? Let Jenna explain the potential causes of smeared DNA. She’ll even give you some tips for getting those crisp bands back.
Standard Protocol for Restriction Enzyme Digests
https://benchfly.com/video/1541/standard-protocol-for-restriction-enzyme-dige/If you’re new to molecular biology, or just need a refresher on best practices for restriction enzyme digests, watch as Dave presents the standard protocol for DNA digestion with restriction enzymes. He include important, but often forgotten, tips for optimal results, including the importance of completely thawed and mixed buffers.
Restriction Enzyme Digest Protocol: Cutting Close to DNA End
https://benchfly.com/video/1542/restriction-enzyme-digest-protocol-cutting-cl/Did you know that proximity to the end of a DNA molecule can influence the success of a restriction enzyme digestion? Well, it can, and Yvette explains how to handle cleavage close to ends. Her tips can prevent you from wasting time and enzyme cutting it too close!
What Causes a Difficult Ligation Reaction
https://benchfly.com/video/1543/what-causes-a-difficult-ligation-reaction/If your DNA fragments have blunt ends or single-base overhangs, you’ll want to watch as Greg explains why they can be difficult to ligate, and how you can improve the outcomes of your ligation reactions. A few simple tips can help you to overcome the challenges posed by ligation of non-cohesive ends.
How to create a comparison graph
https://benchfly.com/video/2331/how-to-create-a-comparison-graph/Making a clear and informative graph in Excel can be daunting, unless you are an Excel guru or have slogged through making hundreds of graphs in the past. Here are a few tips to take the frustration out of making a comparison graph.
