100 Search Results for "chromatography and tlc" – Page 2
Your Pipette and You, Part I
https://benchfly.com/video/175/your-pipette-and-you-part-i/The micropipettor is one of the most used tools in the biochemist's and molecular biologist's toolbox. Learn to use it properly and your data will be so much better!
Restriction Enzyme Digest Problem: Too Many DNA Bands
https://benchfly.com/video/1538/restriction-enzyme-digest-problem-too-many-dn/It has happened to us all; you run a gel expecting one or two bands, but end up with additional, unexpected bands of varying size and intensity. Typically, these off-target bands are caused by either star activity or partial digestion. Yvette explains both of these phenomena, and gives tips for avoiding them in the future, including optimizing incubation time and using NEB’s High-Fidelity (HF®) Restriction Enzymes.
Micropipette Calibration and Analysis
https://benchfly.com/video/112/micropipette-calibration-and-analysis/Proper micropipette calibration is critically important and should be performed annually to ensure volumes are accurate. Over time, pipette volumes tend to drift so the more time passes after calibration, the larger the odds that the pipette is not picking up or dispensing the desired volume. Therefore, micropipette calibration can directly impact the reproducibility of experiments and the quality of the resulting data.
Introduction to Gibson Assembly
https://benchfly.com/video/1547/introduction-to-gibson-assembly/Learn how Gibson Assembly, a new method for assembling DNA fragments, can make your synthetic biology and cloning experiments quicker and easier than ever before. Save time and improve your yields!
Creating an Aspiration Apparatus
https://benchfly.com/video/28/creating-an-aspiration-apparatus/Creating an aspiration apparatus in the hood that has a safety trap and is easy to empty is an important part of good sterile technique. Without the proper equipment, you may end up with a house vac full of liquid and bacteria. So take the time to set up your apparatus right and prevent the hazmat team from shutting you down...
How to Pull Capillary Tubes
https://benchfly.com/video/56/how-to-pull-capillary-tubes/Better learn to pull capillary tubes of your own because your labmates probably won't be too happy if you continue to drain their TLC spotters without replacing them. After a little practice, you'll be able to stockpile a decent supply of capillary tubes in just a few minutes.
How to Pour an Agarose Column
https://benchfly.com/video/731/how-to-pour-an-agarose-column/Pouring an agarose gel may not be the most exciting protocol in the world, but it's a key technique in the lab. Here we show you how to pour highly reproducible gels for technically demanding experiments. We also give tips on shortcuts you can take when pouring routine gels, where precision isn't critical. Buying resin in bulk and packing yourself is fast and is an easy way to save money.
How to Mail DNA
https://benchfly.com/video/30/how-to-mail-dna/Knowing this trick for how to mail DNA to collaborators will put an end to hunts for styrofoam boxes and active FedEx account number. If you have an envelope and a postage stamp, you're ready to get started.
Episode 2: Interview with ex-FDA regulator Dr Audrey Jia
https://benchfly.com/video/3346/episode-2-interview-with-ex-fda-regulator-dr-/Subject matter is "importance and utility of dedicated imaging in cell line development" Dr Jia also touches on some other important topics including legacy cell lines and also drawbacks of the ClonePix for clonality
How to Wetpack a Silica Gel Column
https://benchfly.com/video/92/how-to-wetpack-a-silica-gel-column/Everyone's got their own style to wetpack a silica gel column, but this is how I do it. Fast, easy and reliable- just what I'm looking for...
