100 Search Results for "chromatography and tlc"  – Page 6

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On-Off bike lights using arduino

https://benchfly.com/video/404/on-off-bike-lights-using-arduino/

This is a project done for an introduction to electronics course. It's a really simple project combining a pushbutton, an arduino uno, and some LEDs. The project I meant to do in the first place was to use the LEDs as turn signals but I don't know how to program this ...yet. The main problems I have with programming are incorporating a second push button while making the LEDs blink. For now, this is something I can do successfully. However, I really want to branch off of this so any feedback is sooo very appreciated!!

Tagged as: Arduino, LED

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A new approach to peer review for scientific publishing - how you can publish faster

https://benchfly.com/video/640/a-new-approach-to-peer-review-for-scientific-/

What if authors could get an unbiased, independent, journal-quality peer review in one week? What if the score from that review could match papers to the best-fit journals for faster publication? Watch this video and explore the opportunities of independent peer review from Rubriq (www.rubriq.com).

Tagged as: peer review, scientific publishing, publishing, STM publishing, getting published, independent peer review,

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IPTG Stability- Solid and in Solution

https://benchfly.com/video/781/iptg-stability-solid-and-in-solution/

IPTG stability varies depending on whether it's stored in solid form or in solution.

Tagged as: iptg stability, iptg stability at room temperature, iptg stability in water, iptg storage, iptg storage temperature, iptg storage conditions, iptg storage solution, how long is iptg good for, how long does iptg last, GoldBio

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BaF3 Cell Proliferation Assay

https://benchfly.com/video/1535/baf3-cell-proliferation-assay/

This is how we perform our standard BaF3 cell proliferation assay. Cells either express FGFR1C or FGFR2C and growth is monitored via 3H-thymidine uptake.

Tagged as: mitogenic assay, growth factor, replication, scintillation counter, GoldBio

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Blue-White Screening Explained

https://benchfly.com/video/1719/blue-white-screening-explained/

Blue-white screening is a common molecular biology technique. In this whiteboard session, Patrick walks us through the science behind what creates a blue or white colony and how we can use this to screen bacterial colonies for desired plasmids.

Tagged as: bacteria, bacterial, molecular biology, whiteboard, lacz, iptg, GoldBio

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What is Restriction Enzyme Star Activity?

https://benchfly.com/video/1539/what-is-restriction-enzyme-star-activity-/

Not sure what star activity is or how it can affect your restriction enzyme DNA digests? Learn more as Rick discusses how various contaminants can increase the off-target cleavage that is a hallmark of star activity, and how NEB’s High-Fidelity (HF®) enzymes have been engineered to decrease star activity.

Tagged as: cloning, molecular biology, troubleshooting

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Standard Protocol for Restriction Enzyme Digests

https://benchfly.com/video/1541/standard-protocol-for-restriction-enzyme-dige/

If you’re new to molecular biology, or just need a refresher on best practices for restriction enzyme digests, watch as Dave presents the standard protocol for DNA digestion with restriction enzymes. He include important, but often forgotten, tips for optimal results, including the importance of completely thawed and mixed buffers.

Tagged as: molecular biology, cloning

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What Causes a Difficult Ligation Reaction

https://benchfly.com/video/1543/what-causes-a-difficult-ligation-reaction/

If your DNA fragments have blunt ends or single-base overhangs, you’ll want to watch as Greg explains why they can be difficult to ligate, and how you can improve the outcomes of your ligation reactions. A few simple tips can help you to overcome the challenges posed by ligation of non-cohesive ends.

Tagged as: cloning, molecular biology, ligase

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Preparing a Two Sample Flow Cell

https://benchfly.com/video/167/preparing-a-two-sample-flow-cell/

This video illustrates how to make a double sample flow cell, where 2 microfluidics experiments can be run side-by-side. We use this setup typically in our DNA tethering experiments. The setup is composed of a slide, a cover slip, and double-sided tape.

Tagged as: cell, flow, kinesin, optical tweezers, microfluidics, koch lab

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How to Film a Scientific Video

https://benchfly.com/video/24/how-to-film-a-scientific-video/

Learning to properly film a scientific video is an important skill for modern scientists. Recording laboratory techniques, protocols, and experiments will only become more prevalent as online tools continue to impact research. Here are a few suggestions to help get your film career underway!

Tagged as: how to film, how to shoot, introduction, tips, advice, perspective, lighting, sound, science videos, current protocols, laboratory techniques, benchfly, tip, protocol

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