100 Search Results for "chromatography and tlc" – Page 4
Labratory dillutions for a titration
https://benchfly.com/video/1741/labratory-dillutions-for-a-titration/Often times you need to load different amounts of DNA onto a gel. For a titration for example. Students often struggle with the idea of how to approach this when your solutions are not at the "perfect" concentrations and the fact you have to make choices and they may be wrong. This video helps walk through the process of this type of problem.
How to Perform Colony PCR
https://benchfly.com/video/57/how-to-perform-colony-pcr/Colony PCR is an easy way to rapidly screen ligation reactions for positive clones - and will save you both time and money. Once you try it, this technique will change your molecular biology life.
Restriction Enzyme Digest Protocol: Cutting Close to DNA End
https://benchfly.com/video/1542/restriction-enzyme-digest-protocol-cutting-cl/Did you know that proximity to the end of a DNA molecule can influence the success of a restriction enzyme digestion? Well, it can, and Yvette explains how to handle cleavage close to ends. Her tips can prevent you from wasting time and enzyme cutting it too close!
Why Should You Use PEG in a Ligation Reaction?
https://benchfly.com/video/1544/why-should-you-use-peg-in-a-ligation-reaction/Have you been adding PEG to your ligase reactions, but weren’t entirely sure why? Or did someone recommend the addition of PEG to improve your ligations, but you’d like a little background on why it might help? Greg can explain the hows and whys of PEG’s addition to ligation reactions, and help you to decide whether or not it might be right for your ligations.
How to Keep a Clean Waterbath
https://benchfly.com/video/58/how-to-keep-a-clean-waterbath/Keeping a clean waterbath is an important step in preventing bacterial and fungal contaminations, which can wreak havoc on tissue culture rooms and incubators. This reagent will help keep your waterbath from becoming a slime bath.
Visualizing Starved Worms (C.elegans) Over Time
https://benchfly.com/video/168/visualizing-starved-worms-c-elegans-over-time/Once C.elegans deplete their bacterial food source and enter starvation mode, their appearance will change with length of starvation. Here we visualize starved C.elegans at 2-weeks and 6-weeks of nutrient deprivation to compare phenotypes of both samples.
How to Use a Pipetman
https://benchfly.com/video/151/how-to-use-a-pipetman/Understanding the basic principles of how to use a pipetman is an essential part of biological research. Here's how to select the proper pipetman, load samples, and dispense liquid.
How to Read Volume on a Pipetman
https://benchfly.com/video/152/how-to-read-volume-on-a-pipetman/Reading volume on a pipetman will eventually become second nature, but when first starting out, it can be easy to get tripped up. Here's a quick tutorial on reading the volume in P20, P200 and P1000 pipetman.
How to Access LCMS Data in Analyst Software
https://benchfly.com/video/154/how-to-access-lcms-data-in-analyst-software/After running a sample on the LCMS, here's how to retrieve the spectrum and isolate desired peaks.
How to Setup and Run LCMS Software
https://benchfly.com/video/134/how-to-setup-and-run-lcms-software/You can use this technique to upload a single sample or an entire set on the LCMS.
