98 Search Results for "literature and genome" – Page 3
How to Perform Colony PCR
https://benchfly.com/video/57/how-to-perform-colony-pcr/Colony PCR is an easy way to rapidly screen ligation reactions for positive clones - and will save you both time and money. Once you try it, this technique will change your molecular biology life.
How to Pour an Agarose Column
https://benchfly.com/video/731/how-to-pour-an-agarose-column/Pouring an agarose gel may not be the most exciting protocol in the world, but it's a key technique in the lab. Here we show you how to pour highly reproducible gels for technically demanding experiments. We also give tips on shortcuts you can take when pouring routine gels, where precision isn't critical. Buying resin in bulk and packing yourself is fast and is an easy way to save money.
Pouring and Running an Agarose Gel
https://benchfly.com/video/91/pouring-and-running-an-agarose-gel/Knowing how to pour and run DNA agarose gel electrophoresis is essential for many molecular biology techniques. Here I show how to pour agarose gels in two of the most common types of gel boxes.
Restriction Enzyme Digest Protocol: Cutting Close to DNA End
https://benchfly.com/video/1542/restriction-enzyme-digest-protocol-cutting-cl/Did you know that proximity to the end of a DNA molecule can influence the success of a restriction enzyme digestion? Well, it can, and Yvette explains how to handle cleavage close to ends. Her tips can prevent you from wasting time and enzyme cutting it too close!
Perfecta3D Hanging Drop Plates for 3D Spheroid Culture - Pipetting Demo
https://benchfly.com/video/1262/perfecta3d-hanging-drop-plates-for-3d-spheroi/Perfecta3D Hanging Drop Plates allow researchers to form uniform 3D spheroids or embryoid bodies. In a 96- or 384- well format, users simply pipet their cell suspension into each well, and it hangs in a drop below the well. As cells are in suspension, not in contact with any matrices or surfaces, they self-assemble into a spheroid. One spheroid per well gives researchers control over their data points and the size of each spheroid.
How to Keep a Clean Waterbath
https://benchfly.com/video/58/how-to-keep-a-clean-waterbath/Keeping a clean waterbath is an important step in preventing bacterial and fungal contaminations, which can wreak havoc on tissue culture rooms and incubators. This reagent will help keep your waterbath from becoming a slime bath.
How to Make a Bacterial Spreader
https://benchfly.com/video/65/how-to-make-a-bacterial-spreader/Making your own bacterial spreader is fast and easy as we demonstrate in this video - all you need is a pipette and a flame. No words necessary...it's that easy!
Labratory dillutions for a titration
https://benchfly.com/video/1741/labratory-dillutions-for-a-titration/Often times you need to load different amounts of DNA onto a gel. For a titration for example. Students often struggle with the idea of how to approach this when your solutions are not at the "perfect" concentrations and the fact you have to make choices and they may be wrong. This video helps walk through the process of this type of problem.
Why Should You Use PEG in a Ligation Reaction?
https://benchfly.com/video/1544/why-should-you-use-peg-in-a-ligation-reaction/Have you been adding PEG to your ligase reactions, but weren’t entirely sure why? Or did someone recommend the addition of PEG to improve your ligations, but you’d like a little background on why it might help? Greg can explain the hows and whys of PEG’s addition to ligation reactions, and help you to decide whether or not it might be right for your ligations.
Blue-White Screening Explained
https://benchfly.com/video/1719/blue-white-screening-explained/Blue-white screening is a common molecular biology technique. In this whiteboard session, Patrick walks us through the science behind what creates a blue or white colony and how we can use this to screen bacterial colonies for desired plasmids.
