98 Search Results for "literature and genome" – Page 4
Important Tips for Working with Q5 Polymerase
https://benchfly.com/video/1546/important-tips-for-working-with-q5-polymerase/Are you using Q5 High-Fidelity DNA Polymerase? Then you probably already know how robust and accurate the DNA amplification is (>100X Taq DNA Polymerase). Melanie has some tips for ensuring optimal performance with Q5. If you haven’t tried Q5 yet, what are you waiting for?
Using an Autoclave
https://benchfly.com/video/139/using-an-autoclave/Information on how to use an autoclave for typical molecular biology reagents and supplies.
Gel Red vs EtBr
https://benchfly.com/video/158/gel-red-vs-etbr/Here we compare the DNA stains Gel-red vs. EtBr head to head. The Gel-red is in sample whereas the EtBr is in gel. All conditions are the same except for the DNA stains. I am using 1X TAE buffer and a VWR agarose.
How to Setup and Run LCMS Software
https://benchfly.com/video/134/how-to-setup-and-run-lcms-software/You can use this technique to upload a single sample or an entire set on the LCMS.
How to Access LCMS Data in Analyst Software
https://benchfly.com/video/154/how-to-access-lcms-data-in-analyst-software/After running a sample on the LCMS, here's how to retrieve the spectrum and isolate desired peaks.
How to Use a Pipetman
https://benchfly.com/video/151/how-to-use-a-pipetman/Understanding the basic principles of how to use a pipetman is an essential part of biological research. Here's how to select the proper pipetman, load samples, and dispense liquid.
How to Read Volume on a Pipetman
https://benchfly.com/video/152/how-to-read-volume-on-a-pipetman/Reading volume on a pipetman will eventually become second nature, but when first starting out, it can be easy to get tripped up. Here's a quick tutorial on reading the volume in P20, P200 and P1000 pipetman.
What Causes a Difficult Ligation Reaction
https://benchfly.com/video/1543/what-causes-a-difficult-ligation-reaction/If your DNA fragments have blunt ends or single-base overhangs, you’ll want to watch as Greg explains why they can be difficult to ligate, and how you can improve the outcomes of your ligation reactions. A few simple tips can help you to overcome the challenges posed by ligation of non-cohesive ends.
How to Remove Bubbles When Pouring LB-Agar Plates
https://benchfly.com/video/165/how-to-remove-bubbles-when-pouring-lb-agar-pl/Here's an easy way to remove bubbles from LB-agar plates. Bubbles should be avoided because they can make spreading bacteria and identifying colonies difficult.
Standard Protocol for Restriction Enzyme Digests
https://benchfly.com/video/1541/standard-protocol-for-restriction-enzyme-dige/If you’re new to molecular biology, or just need a refresher on best practices for restriction enzyme digests, watch as Dave presents the standard protocol for DNA digestion with restriction enzymes. He include important, but often forgotten, tips for optimal results, including the importance of completely thawed and mixed buffers.
