97 Search Results for "gel and acrylamide and agarose" – Page 2
Introduction to Gibson Assembly
https://benchfly.com/video/1547/introduction-to-gibson-assembly/Learn how Gibson Assembly, a new method for assembling DNA fragments, can make your synthetic biology and cloning experiments quicker and easier than ever before. Save time and improve your yields!
Your Pipette and You, Part I
https://benchfly.com/video/175/your-pipette-and-you-part-i/The micropipettor is one of the most used tools in the biochemist's and molecular biologist's toolbox. Learn to use it properly and your data will be so much better!
Micropipette Calibration and Analysis
https://benchfly.com/video/112/micropipette-calibration-and-analysis/Proper micropipette calibration is critically important and should be performed annually to ensure volumes are accurate. Over time, pipette volumes tend to drift so the more time passes after calibration, the larger the odds that the pipette is not picking up or dispensing the desired volume. Therefore, micropipette calibration can directly impact the reproducibility of experiments and the quality of the resulting data.
How to Cool LB-Agar Quickly
https://benchfly.com/video/161/how-to-cool-lb-agar-quickly/After autoclaving, you want to cool LB agar quickly and evenly before adding antibiotic (optional) and pouring the plates. Here's an easy way to bring the temp down quickly so you can pour your plates and get on with your day!
Creating an Aspiration Apparatus
https://benchfly.com/video/28/creating-an-aspiration-apparatus/Creating an aspiration apparatus in the hood that has a safety trap and is easy to empty is an important part of good sterile technique. Without the proper equipment, you may end up with a house vac full of liquid and bacteria. So take the time to set up your apparatus right and prevent the hazmat team from shutting you down...
Labratory dillutions for a titration
https://benchfly.com/video/1741/labratory-dillutions-for-a-titration/Often times you need to load different amounts of DNA onto a gel. For a titration for example. Students often struggle with the idea of how to approach this when your solutions are not at the "perfect" concentrations and the fact you have to make choices and they may be wrong. This video helps walk through the process of this type of problem.
Reusing Agarose Gels
https://benchfly.com/video/108/reusing-agarose-gels/This is how I reuse agarose gels for my genotyping since I run large numbers of samples every few days - saving me time and money.
DNA Horizontal Gel Electrophoresis
https://benchfly.com/video/110/dna-horizontal-gel-electrophoresis/Here's a tutorial on how to perform horizontal DNA gel electrophoresis. This is one of the most common biochemical techniques used to separate and analyze DNA.
Pouring Acrylamide Gels for SDS-PAGE
https://benchfly.com/video/106/pouring-acrylamide-gels-for-sds-page/Here's how to pour acrylamide gels for SDS-PAGE using a BioRad system. Although the plates may vary with other systems, the technique and recipes for pouring acrylamide gels will still be applicable.
How to Bomb-load Capillary Tips or Frits for LC/MS
https://benchfly.com/video/104/how-to-bomb-load-capillary-tips-or-frits-for-/This is how we prepare and bomb load capillary tips and frits for use in LC/MS. Be sure to wear proper protection as this protocol uses high pressure to pack the LC/MS tips.
