97 Search Results for "gel and acrylamide and agarose" – Page 6
Blue-White Screening Explained
https://benchfly.com/video/1719/blue-white-screening-explained/Blue-white screening is a common molecular biology technique. In this whiteboard session, Patrick walks us through the science behind what creates a blue or white colony and how we can use this to screen bacterial colonies for desired plasmids.
What is Restriction Enzyme Star Activity?
https://benchfly.com/video/1539/what-is-restriction-enzyme-star-activity-/Not sure what star activity is or how it can affect your restriction enzyme DNA digests? Learn more as Rick discusses how various contaminants can increase the off-target cleavage that is a hallmark of star activity, and how NEB’s High-Fidelity (HF®) enzymes have been engineered to decrease star activity.
Standard Protocol for Restriction Enzyme Digests
https://benchfly.com/video/1541/standard-protocol-for-restriction-enzyme-dige/If you’re new to molecular biology, or just need a refresher on best practices for restriction enzyme digests, watch as Dave presents the standard protocol for DNA digestion with restriction enzymes. He include important, but often forgotten, tips for optimal results, including the importance of completely thawed and mixed buffers.
What Causes a Difficult Ligation Reaction
https://benchfly.com/video/1543/what-causes-a-difficult-ligation-reaction/If your DNA fragments have blunt ends or single-base overhangs, you’ll want to watch as Greg explains why they can be difficult to ligate, and how you can improve the outcomes of your ligation reactions. A few simple tips can help you to overcome the challenges posed by ligation of non-cohesive ends.
Important Tips for Working with Q5 Polymerase
https://benchfly.com/video/1546/important-tips-for-working-with-q5-polymerase/Are you using Q5 High-Fidelity DNA Polymerase? Then you probably already know how robust and accurate the DNA amplification is (>100X Taq DNA Polymerase). Melanie has some tips for ensuring optimal performance with Q5. If you haven’t tried Q5 yet, what are you waiting for?
How to create a comparison graph
https://benchfly.com/video/2331/how-to-create-a-comparison-graph/Making a clear and informative graph in Excel can be daunting, unless you are an Excel guru or have slogged through making hundreds of graphs in the past. Here are a few tips to take the frustration out of making a comparison graph.
Top 5 Reasons to Use a Hypothermic Preservation Additive
https://benchfly.com/video/2751/top-5-reasons-to-use-a-hypothermic-preservati/Short video about the top 5 reasons why our market likes to use a hypothermic preservation additive like ROKEPIE. Showing application and benefits.
Serra do Ramalho, Gruta da Pingueira II
https://benchfly.com/video/7292/serra-do-ramalho-gruta-da-pingueira-ii/Foraging and interaction behavior of a new amphibian species of Xangoniscus (Oniscidea, Styloniscidae).
How to Read Volume on a Pipetman
https://benchfly.com/video/152/how-to-read-volume-on-a-pipetman/Reading volume on a pipetman will eventually become second nature, but when first starting out, it can be easy to get tripped up. Here's a quick tutorial on reading the volume in P20, P200 and P1000 pipetman.
How to Film a Scientific Video
https://benchfly.com/video/24/how-to-film-a-scientific-video/Learning to properly film a scientific video is an important skill for modern scientists. Recording laboratory techniques, protocols, and experiments will only become more prevalent as online tools continue to impact research. Here are a few suggestions to help get your film career underway!
