97 Search Results for "gel and acrylamide and agarose" – Page 4
Perfecta3D Hanging Drop Plates for 3D Spheroid Culture - Pipetting Demo
https://benchfly.com/video/1262/perfecta3d-hanging-drop-plates-for-3d-spheroi/Perfecta3D Hanging Drop Plates allow researchers to form uniform 3D spheroids or embryoid bodies. In a 96- or 384- well format, users simply pipet their cell suspension into each well, and it hangs in a drop below the well. As cells are in suspension, not in contact with any matrices or surfaces, they self-assemble into a spheroid. One spheroid per well gives researchers control over their data points and the size of each spheroid.
Why Should You Use PEG in a Ligation Reaction?
https://benchfly.com/video/1544/why-should-you-use-peg-in-a-ligation-reaction/Have you been adding PEG to your ligase reactions, but weren’t entirely sure why? Or did someone recommend the addition of PEG to improve your ligations, but you’d like a little background on why it might help? Greg can explain the hows and whys of PEG’s addition to ligation reactions, and help you to decide whether or not it might be right for your ligations.
Restriction Enzyme Digest Protocol: Cutting Close to DNA End
https://benchfly.com/video/1542/restriction-enzyme-digest-protocol-cutting-cl/Did you know that proximity to the end of a DNA molecule can influence the success of a restriction enzyme digestion? Well, it can, and Yvette explains how to handle cleavage close to ends. Her tips can prevent you from wasting time and enzyme cutting it too close!
How to Quickly Pour a Small Number of LB-Agar Plates
https://benchfly.com/video/160/how-to-quickly-pour-a-small-number-of-lb-agar/If you want to quickly pour LB agar plates, but you only need a small number of them, preparing, autoclaving, pouring and cooling a large batch can be time consuming and wasteful. Here's a quick tip to pour a small number of plates quickly.
Restriction Enzyme Digest Problem: Smear on Agarose Gel
https://benchfly.com/video/1540/restriction-enzyme-digest-problem-smear-on-ag/You’re expecting a band at 89 kD, but all you see is an uninterpretable smear when you analyze your agarose gel. What went wrong? Let Jenna explain the potential causes of smeared DNA. She’ll even give you some tips for getting those crisp bands back.
How to Load and Run SDS-PAGE Gels
https://benchfly.com/video/120/how-to-load-and-run-sds-page-gels/Proper SDS-PAGE gel loading technique can prevent the tragedy of performing an entire experiment only to have your samples splash into adjacent wells just a couple of hours away from the result.
Preventing Leaks When Pouring Plates for SDS-PAGE
https://benchfly.com/video/119/preventing-leaks-when-pouring-plates-for-sds-/Old glass SDS-PAGE plates can get chipped and cracked- here's an easy way to continue to use them without the leaks.
DNA Electrophoresis Sample Loading Examples
https://benchfly.com/video/111/dna-electrophoresis-sample-loading-examples/Just a quick video showing how to load a horizontal DNA electrophoresis gel. The tutorial includes a close-up of the loading and some common mistakes when loading.
A Tip for Loading the Ladder in SDS-PAGE
https://benchfly.com/video/105/a-tip-for-loading-the-ladder-in-sds-page/No need to dial the pipetman back and forth between the extreme volumes - here's an easy way to load the right amount of SDS-PAGE ladder without changing volume at all!
Cotton Trick for Column Chromatography
https://benchfly.com/video/94/cotton-trick-for-column-chromatography/No more messing around with a stick to get the cotton or glass wool positioned properly when preparing for column chromatography. Use this trick and you'll be done in 5 seconds.
