110 Search Results for "cell culture and tissue culture"  – Page 10

How to Wetpack a Silica Gel Column

https://benchfly.com/video/92/how-to-wetpack-a-silica-gel-column/

Everyone's got their own style to wetpack a silica gel column, but this is how I do it. Fast, easy and reliable- just what I'm looking for...

Tagged as: chromatography, pack, pour, science videos, current protocols, laboratory techniques, benchfly, chemistry, chemical biology

Tissue Prep for LCMS - Pt.6: Cutting ZOOM Strips

https://benchfly.com/video/117/tissue-prep-for-lcms--pt-6-cutting-zoom-strip/

After isoelectric focusing, we cut ZOOM strips containing the separated peptide fragments for further analysis, as shown here.

Tagged as: laboratory techniques, science videos, current protocols, proteomics, protein, peptide separation, Isoelectric Focusing, IEF, spectrometry, mass spec

siRNA transfection tip

https://benchfly.com/video/73/sirna-transfection-tip/

This is now my standard protocol for siRNA transfection. I found it increases the efficiency of knockdown, which can really improve cell-based experiments.

Tagged as: sirna, trick, laboratory techniques, current protocols, biology, biochemistry, chemical biology, rna, molecular biology

Centrifuge Problem, Overtemp

https://benchfly.com/video/42/centrifuge-problem-overtemp/

If your centrifuge is over temp, follow these steps to diagnose the problem and see if you need to call for service.

Tagged as: temperature error, won\'t cool, troubleshooting, compressor not turning on, instrument, repair, laboratory techniques, current protocols, fault

How to Smear Parasites for Counting

https://benchfly.com/video/123/how-to-smear-parasites-for-counting/

Here is our method for preparing a cells to quantify parasitemia in red blood cells.

Tagged as: plasmodium, falciparum, microscope, slide, laboratory techniques, science videos, current protocols, biology, cell biology,

How to Read Volume on a Pipetman

https://benchfly.com/video/152/how-to-read-volume-on-a-pipetman/

Reading volume on a pipetman will eventually become second nature, but when first starting out, it can be easy to get tripped up. Here's a quick tutorial on reading the volume in P20, P200 and P1000 pipetman.

Tagged as: P20, P200, P100, pipetman, laboratory techniques, science videos, current protocols, micropipette, biology, biochemistry, molecular biology

How to Use a Pipetman

https://benchfly.com/video/151/how-to-use-a-pipetman/

Understanding the basic principles of how to use a pipetman is an essential part of biological research. Here's how to select the proper pipetman, load samples, and dispense liquid.

Tagged as: pipetman, pipetteman, laboratory techniques, science videos, current protocols, biology, biochemistry, introduction, beginner, molecular biology

Centrifuge problem, speed and tach errors

https://benchfly.com/video/43/centrifuge-problem-speed-and-tach-errors/

If your instrument is showing a speed or tach error, there are a couple of things to check that may help fix the problem.

Tagged as: tach error, ultracentrifuge, troubleshooting, centrifuge broken, repair, instrument, laboratory techniques, current protocols, fault

How to Access LCMS Data in Analyst Software

https://benchfly.com/video/154/how-to-access-lcms-data-in-analyst-software/

After running a sample on the LCMS, here's how to retrieve the spectrum and isolate desired peaks.

Tagged as: analyst, lcms, proteomics, laboratory techniques, science videos, current protocols, mass spec, spectrometer

How to Setup and Run LCMS Software

https://benchfly.com/video/134/how-to-setup-and-run-lcms-software/

You can use this technique to upload a single sample or an entire set on the LCMS.

Tagged as: lc/ms, mass spec, spectrometry, laboratory techniques, current protocols, science videos,