110 Search Results for "cell culture and tissue culture"  – Page 3

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Interview with ex-FDA regulator : Session 1 (of 4)

https://benchfly.com/video/3093/interview-with-ex-fda-regulator-session-1-of-/

Exclusive interview with ex-FDA reviewer Dr Audrey Jia conducted in September 2015. In this session, Dr Jia answers questions on "Current perspectives on clonality in cell line development"

Tagged as: FDA, compliance, regulator, clonality, cell line development, single cell cloning

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Restriction Enzyme Digest Problem: Too Many DNA Bands

https://benchfly.com/video/1538/restriction-enzyme-digest-problem-too-many-dn/

It has happened to us all; you run a gel expecting one or two bands, but end up with additional, unexpected bands of varying size and intensity. Typically, these off-target bands are caused by either star activity or partial digestion. Yvette explains both of these phenomena, and gives tips for avoiding them in the future, including optimizing incubation time and using NEB’s High-Fidelity (HF®) Restriction Enzymes.

Tagged as: troubleshooting, molecular biology, cloning,

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Introduction to Gibson Assembly

https://benchfly.com/video/1547/introduction-to-gibson-assembly/

Learn how Gibson Assembly, a new method for assembling DNA fragments, can make your synthetic biology and cloning experiments quicker and easier than ever before. Save time and improve your yields!

Tagged as: molecular biology, cloning, ligation, ligase

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Streaking Bacteria for a Single Colony

https://benchfly.com/video/126/streaking-bacteria-for-a-single-colony/

When streaking bacteria, it's important to use a technique that will ensure single colonies can be isolated 8-12 hours later. This video demonstrates how to streak bacteria from a stock in a manner that spreads the bacteria over a broad concentration range.

Tagged as: bacterial, culture, "laboratory techniques", science videos, current protocols, biology, cloning, molecular biology, biochemistry, dna

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Tissue Prep for LCMS - Pt.1: Sonication

https://benchfly.com/video/115/tissue-prep-for-lcms--pt-1-sonication/

Tissue sonication is the first step in preparing samples for LCMS analysis. Here we walk you through the process.

Tagged as: lc/ms, proteomics, mass spec, spectrometry, ayers, protein, sample, laboratory techniques, current protocols, science videos, sonicate, sonication,

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Tissue Prep for LCMS - Pt.8: Final Desalting

https://benchfly.com/video/133/tissue-prep-for-lcms--pt-8-final-desalting/

Peptide desalting is performed on the purified, digested sample in the final step of preparing the tissue sample for LCMS analysis.

Tagged as: lc/ms, proteomics, mass spec, spectrometry, ayers, protein, sample, laboratory techniques, current protocols, science videos, C18, sep pak, proteomics, mass spectrometer,

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Serial Dilution

https://benchfly.com/video/113/serial-dilution/

Performing a proper serial dilution is important when preparing stock reagents and performing experiments. Here is the technique and the math behind serial dilution.

Tagged as: Serial, dilution, kirkwood, greg, calculation, pipette

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How to Use Graduated Cylinders

https://benchfly.com/video/153/how-to-use-graduated-cylinders/

Learning how to use graduated cylinders to accurately read volume is an essential component of biological and chemical research. So here's a quick tutorial on how to select and read the properly sized graduated cylinder for your experiment.

Tagged as: graduated cylinders, volume, laboratory techniques, science videos, current protocols, chemistry, biology, biochemistry, measure

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How to Pour an Agarose Column

https://benchfly.com/video/731/how-to-pour-an-agarose-column/

Pouring an agarose gel may not be the most exciting protocol in the world, but it's a key technique in the lab. Here we show you how to pour highly reproducible gels for technically demanding experiments. We also give tips on shortcuts you can take when pouring routine gels, where precision isn't critical. Buying resin in bulk and packing yourself is fast and is an easy way to save money.

Tagged as: column, agarose, protein, GoldBio, DNA

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