110 Search Results for "cell culture and tissue culture"  – Page 7

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Swinging Bucket Rotor Care

https://benchfly.com/video/40/swinging-bucket-rotor-care/

Perform routine swinging bucket rotor care to ensure proper function and avoid a costly malfunction.

Tagged as: bucket pins, imbalance, tabletop, centrifuge, repair, instrument, troubleshooting, laboratory techniques, current protocols

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How to Leave a Comment

https://benchfly.com/video/72/how-to-leave-a-comment/

This is the easiest way to leave a comment on BenchFly. Set it up once in under a minute and you won't have to login ever again!

Tagged as: intensedebate, intense debate, demo, screenshot, science videos, current protocols, laboratory techniques, benchfly

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What is Restriction Enzyme Star Activity?

https://benchfly.com/video/1539/what-is-restriction-enzyme-star-activity-/

Not sure what star activity is or how it can affect your restriction enzyme DNA digests? Learn more as Rick discusses how various contaminants can increase the off-target cleavage that is a hallmark of star activity, and how NEB’s High-Fidelity (HF®) enzymes have been engineered to decrease star activity.

Tagged as: cloning, molecular biology, troubleshooting

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Standard Protocol for Restriction Enzyme Digests

https://benchfly.com/video/1541/standard-protocol-for-restriction-enzyme-dige/

If you’re new to molecular biology, or just need a refresher on best practices for restriction enzyme digests, watch as Dave presents the standard protocol for DNA digestion with restriction enzymes. He include important, but often forgotten, tips for optimal results, including the importance of completely thawed and mixed buffers.

Tagged as: molecular biology, cloning

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What Causes a Difficult Ligation Reaction

https://benchfly.com/video/1543/what-causes-a-difficult-ligation-reaction/

If your DNA fragments have blunt ends or single-base overhangs, you’ll want to watch as Greg explains why they can be difficult to ligate, and how you can improve the outcomes of your ligation reactions. A few simple tips can help you to overcome the challenges posed by ligation of non-cohesive ends.

Tagged as: cloning, molecular biology, ligase

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Important Tips for Working with Q5 Polymerase

https://benchfly.com/video/1546/important-tips-for-working-with-q5-polymerase/

Are you using Q5 High-Fidelity DNA Polymerase? Then you probably already know how robust and accurate the DNA amplification is (>100X Taq DNA Polymerase). Melanie has some tips for ensuring optimal performance with Q5. If you haven’t tried Q5 yet, what are you waiting for?

Tagged as: molecular biology, PCR, cloning

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How to Remove Static from a Scale

https://benchfly.com/video/55/how-to-remove-static-from-a-scale/

Scale acting like it has a mind of it's own? It might be static - try this simple technique to remove static from a scale and tame the beast.

Tagged as: static gun, static, scale, won\'t tare, tear, troubleshooting, science videos, current protocols, laboratory techniques, benchfly, chemistry, biology, instrument, weight

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How to create a comparison graph

https://benchfly.com/video/2331/how-to-create-a-comparison-graph/

Making a clear and informative graph in Excel can be daunting, unless you are an Excel guru or have slogged through making hundreds of graphs in the past. Here are a few tips to take the frustration out of making a comparison graph.

Tagged as: graph making, Excel, error bars

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How to Weigh Small Amounts

https://benchfly.com/video/54/how-to-weigh-small-amounts/

Using the right technique to weigh small amounts of a solid can dramatically improve the accuracy of your readings. The size of both the weigh vessel and the scale should be carefully considered to maximize the quality of your data.

Tagged as: weigh paper, scale, science videos, current protocols, laboratory techniques, benchfly, chemistry, biology, biochemistry

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Changing Tanks on a Gas Manifold

https://benchfly.com/video/32/changing-tanks-on-a-gas-manifold/

High pressure requires high attention... So next time you're changing gas tanks on a manifold, follow these steps for a safe and easy transfer.

Tagged as: co2 tanks, changing gas tank, change tank, manifold, gas, science videos, current protocols, laboratory techniques, benchfly, biology, biochemistry, chemistry, tank,

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