110 Search Results for "cell culture and tissue culture" – Page 4
How to Quickly Pour a Small Number of LB-Agar Plates
https://benchfly.com/video/160/how-to-quickly-pour-a-small-number-of-lb-agar/If you want to quickly pour LB agar plates, but you only need a small number of them, preparing, autoclaving, pouring and cooling a large batch can be time consuming and wasteful. Here's a quick tip to pour a small number of plates quickly.
Why Should You Use PEG in a Ligation Reaction?
https://benchfly.com/video/1544/why-should-you-use-peg-in-a-ligation-reaction/Have you been adding PEG to your ligase reactions, but weren’t entirely sure why? Or did someone recommend the addition of PEG to improve your ligations, but you’d like a little background on why it might help? Greg can explain the hows and whys of PEG’s addition to ligation reactions, and help you to decide whether or not it might be right for your ligations.
Visualizing Starved Worms (C.elegans) Over Time
https://benchfly.com/video/168/visualizing-starved-worms-c-elegans-over-time/Once C.elegans deplete their bacterial food source and enter starvation mode, their appearance will change with length of starvation. Here we visualize starved C.elegans at 2-weeks and 6-weeks of nutrient deprivation to compare phenotypes of both samples.
Restriction Enzyme Digest Protocol: Cutting Close to DNA End
https://benchfly.com/video/1542/restriction-enzyme-digest-protocol-cutting-cl/Did you know that proximity to the end of a DNA molecule can influence the success of a restriction enzyme digestion? Well, it can, and Yvette explains how to handle cleavage close to ends. Her tips can prevent you from wasting time and enzyme cutting it too close!
Labratory dillutions for a titration
https://benchfly.com/video/1741/labratory-dillutions-for-a-titration/Often times you need to load different amounts of DNA onto a gel. For a titration for example. Students often struggle with the idea of how to approach this when your solutions are not at the "perfect" concentrations and the fact you have to make choices and they may be wrong. This video helps walk through the process of this type of problem.
How to Prepare a Slide for the Light Microscope
https://benchfly.com/video/170/how-to-prepare-a-slide-for-the-light-microsco/Here's how to prepare a liquid sample for viewing under a light microscope.
How to Bomb-load Capillary Tips or Frits for LC/MS
https://benchfly.com/video/104/how-to-bomb-load-capillary-tips-or-frits-for-/This is how we prepare and bomb load capillary tips and frits for use in LC/MS. Be sure to wear proper protection as this protocol uses high pressure to pack the LC/MS tips.
Tissue Prep for LCMS - Pt.2: BCA Assay
https://benchfly.com/video/118/tissue-prep-for-lcms--pt-2-bca-assay/After sonicating the tissue, use a BCA assay to quantify the total protein in the sample before digesting it when preparing a sample for LCMS.
How to Perform Colony PCR
https://benchfly.com/video/57/how-to-perform-colony-pcr/Colony PCR is an easy way to rapidly screen ligation reactions for positive clones - and will save you both time and money. Once you try it, this technique will change your molecular biology life.
How to Make a Bacterial Spreader
https://benchfly.com/video/65/how-to-make-a-bacterial-spreader/Making your own bacterial spreader is fast and easy as we demonstrate in this video - all you need is a pipette and a flame. No words necessary...it's that easy!
