110 Search Results for "cell culture and tissue culture" – Page 2
Episode 2: Interview with ex-FDA regulator Dr Audrey Jia
https://benchfly.com/video/3346/episode-2-interview-with-ex-fda-regulator-dr-/Subject matter is "importance and utility of dedicated imaging in cell line development" Dr Jia also touches on some other important topics including legacy cell lines and also drawbacks of the ClonePix for clonality
Preparing a Flow Cell
https://benchfly.com/video/166/preparing-a-flow-cell/This video illustrates the preparation of a single channel flow cell. The cell is made up of a slide, a cover slip, and two pieces of double-sided tape. Liquids can be flowed into the chamber via capillary action. Excess liquied can be swept up with a kim wipe.
Preparing a Two Sample Flow Cell
https://benchfly.com/video/167/preparing-a-two-sample-flow-cell/This video illustrates how to make a double sample flow cell, where 2 microfluidics experiments can be run side-by-side. We use this setup typically in our DNA tethering experiments. The setup is composed of a slide, a cover slip, and double-sided tape.
OpenWetWare Open Notebook
https://benchfly.com/video/173/openwetware-open-notebook/Open Notebook Science is an up and coming aspect to working in the lab. Every lab has their own method of sharing research and we at the KochLab use OpenWetWare.org's notebook feature to make all of our protocols and data public. Here is how I organize and share my notes using all the web has to offer.
Graphing your standards from a protein gel
https://benchfly.com/video/1536/graphing-your-standards-from-a-protein-gel/Basically, how do you create a standard sizing curve from a protein gel. You need to measure the mobility of your bands and graph them. The curve fit is certainly things people argue over, as the relationship is really sigmodial and doesn't fit very well at the extremes but try out different fits and prove to yourself that exponential and power fits are very similar in output.
Micropipette Calibration and Analysis
https://benchfly.com/video/112/micropipette-calibration-and-analysis/Proper micropipette calibration is critically important and should be performed annually to ensure volumes are accurate. Over time, pipette volumes tend to drift so the more time passes after calibration, the larger the odds that the pipette is not picking up or dispensing the desired volume. Therefore, micropipette calibration can directly impact the reproducibility of experiments and the quality of the resulting data.
Tissue Prep for LCMS - Pt.3: Alkylation/Digestion
https://benchfly.com/video/129/tissue-prep-for-lcms--pt-3-alkylation-digesti/Peptide alkylation and digestion are performed is In the third step of tissue preparation for LCMS.
How to Cool LB-Agar Quickly
https://benchfly.com/video/161/how-to-cool-lb-agar-quickly/After autoclaving, you want to cool LB agar quickly and evenly before adding antibiotic (optional) and pouring the plates. Here's an easy way to bring the temp down quickly so you can pour your plates and get on with your day!
BaF3 Cell Proliferation Assay
https://benchfly.com/video/1535/baf3-cell-proliferation-assay/This is how we perform our standard BaF3 cell proliferation assay. Cells either express FGFR1C or FGFR2C and growth is monitored via 3H-thymidine uptake.
Your Pipette and You, Part I
https://benchfly.com/video/175/your-pipette-and-you-part-i/The micropipettor is one of the most used tools in the biochemist's and molecular biologist's toolbox. Learn to use it properly and your data will be so much better!
